Anti Fibronectin (bovine)
Mouse monoclonal antibody
Cat.No. CSI 005-17
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).
Lysed bovine corneal endothelial cells and extracellular matrix.
CSI 005-17 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin).
CSI 005-17 cross-reacts with human and chicken fibronectin. Other species have not been tested.
Epitope is located in the 120kD cell binding fragment.
CSI 005-17 can be used in ELISA, Western blotting, immunoprecipitation and immunostaining of frozen PLP-fixed sections of bovine and human tissues. The antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. It can be used to probe fibronectin conformation. Strong reaction is seen in ELISA with thrombospondin directly coated onto the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:30,000 | 1,2,3,4,5,6 |
| Immunoblotting | Yes | 1:100 | 1 |
| Immunohistochemistry | Yes |
1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.
2. Underwood PA, Steele JG, Dalton BA (1993) Effects of polystyrene surface chemistry on biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies. J Cell Sci 104:793-803.
3. Di Girolamo N, Underwood PA, McCluskey PJ, Wakefield D (1993) Functional activity of plasma fibronectin in patients with Diabetes mellitis. Diabetes 42:1606-1613.
4. Dalton BA, McFarland CD, Underwood PA, Steele JG (1995) Role of heparin binding domain of fibronectin in attachment and spreading of human bone derived cells. J Cell Sci 108:2083-2092.
5. Underwood PA, Bean PA, Mitchell SM, Whitelock JM (2001) Specific affinity depletion of cell adhesion molecules and growth factors from serum. J Immunol Methods 247:217-224.
6. Underwood PA, Steele JG, Dalton BA, Bennett FA (1990) Solid phase monoclonal antibodies. A novel method of directing the function of biologically active molecules by presenting a specific orientation. J Immunol Methods 127:91-102.
Downloads
CSI 005-17.pdf (20.41 kB) Product specification
Cell biology flyer.pdf (199.13 kB)
Cell biology flyer
Related Articles
Antibody MSDS
April 23 2008
